• February 23, 2024

Gentaur Research

Gentaur Research use antibodies for studying proteins interactions:

Immunohistochemistry (IHC):

  • Primary Antibodies: These antibodies directly attach to the target protein within tissue samples.
  • Secondary Antibodies: Theses antibodies bind to the primary antibody and are often linked with enzymes or fluorophores for signal detection.

Western Blotting (WB):

  • Primary Antibodies: Employed to identify specific proteins within a mixture separated by gel electrophoresis.
  • Secondary Antibodies: Linked with enzymes or fluorophores to aid visualization.

Chromatin Immunoprecipitation (ChIP):

  • ChIP-Grade Antibodies: Detected and validated specifically for ChIP experiments, these antibodies should recognize the target protein within its native chromatin context.
  • Control Antibodies: Serve as negative controls in ChIP experiments.

Immunoprecipitation (IP):

  • Protein A or G Conjugated Beads: Commonly used in immunoprecipitation assays to pull down the target protein and its associated partners.
  • Primary Antibodies: Used to selectively capture the protein of interest.

Flow Cytometry (FACS/Flow):

  • Fluorophore-Conjugated Antibodies: Directly labeled with fluorophores, these antibodies help identify and quantify specific proteins on cell surfaces or within cells.
  • Isotype Controls: Used as controls to distinguish specific staining from non-specific background.

Flow cytometry Protein G Antibody

 

It is crucial for researchers to carefully select antibodies for each technique, considering factors such as specificity, sensitivity, and compatibility with experimental conditions. Additionally, incorporating appropriate controls, including isotype controls and negative controls, is essential to ensure the reliability of the results.

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